Correlative Light and Electron Microscopy(CLEM) Seminar 2023
～The world of cell observation by CLEM～

Outline

In this seminar, Dr. Isei Tanida, Senior Associate Professor, Graduate School of Medicine, Juntendo University, will introduce a series of principles and applications of in-resin CLEM.
He will also introduce the latest in-resin CLEM such as Immuno-in-resin CLEM using antibodies.
If you are interested in CLEM, please take this opportunity to attend!

DATE:
Thursday, November 9, 2023

TIME:
7:00 a.m. PST / 10:00 a.m. EST / 15:00 GMT / 16:00 CET / 23:00 CST / 24:00 JST

Duration:
35 Minutes

Registration fee:
Free

Speaker:
Dr. Isei Tanida
Department of Cellular and Molecular Neuropathology, Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine

Title:
Recent Progress in In-resin CLEM: From Cultured Cells to Glial Cells in the CNS

Abstract:
Correlative Light and Electron Microscopy (CLEM) is a technique that allows correlation of fluorescent and electron microscopic images. In-resin CLEM is a powerful method that improves correlation accuracy by observing the same resin-embedded ultrathin sections with both fluorescence and electron microscopy. This approach ensures high correlation except for differences in electron beam and fluorescence wavelengths, while improving the Z-axis resolution of the fluorescence signal. In this presentation, we will introduce several techniques of in-resin CLEM. First, we will show in-resin CLEM using fluorescent proteins such as CLEM-Red and CLEM-Green. Next, we will present in-resin CLEM using proximity labeling with biotin ligase. In addition, we will show our newly developed “immuno-in-resin CLEM”, which is a method to apply immunological reactions to in-resin CLEM of Epon resin-embedded cultured cells. Finally, we will demonstrate the application of immuno-in-resin CLEM using anti-Iba1 and anti-GFAP antibodies on microglia and astrocytes in the CNS of a mouse model of neuronal ceroid lipofuscinosis. This application shed light on ultrastructural abnormalities of microglia and astrocytes in the CNS of a mouse brain model of neurodegenerative disease.

References:
(1) Mitsui et al. Glia, 2023, (2) Tanida et al. Heliyon. 9(6):e17394, 2023, (3) Tanida et al. Methods Mol Biol. 2564:287-297, 2023, (4) Sanada et al. Sci Rep. 12:11130, 2022

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